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In a triplex (3-Plex) fluorescence amplification system
The results showed that the melting curve of the SYBR Green Blue qPCR Mix displayed a standard single peak
Its fidelity is approximately 100 times that of regular Taq DNA polymerase
Amplification of complex template
Hotstart KnTaq Polymerase is an N-truncated Taq DNA polymerase with a stable hot-start system and no 5'-3' exonuclease activity
Klenow Fragment（3´→5´ exo-） Spec.:K010-C/1,000 U In a triplex (3-Plex) fluorescenceKlenow Fragment 35 exo is an N terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5 3 exonuclease activity and has mutations which abolish the 35 exonuclease activity. Application 1. Generates probes using random primers 2. Random priming labeling 3. dA tailing at 3' end 4. Second strand cDNA synthesis Features Moderate strand displacement activity
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